BIOLOGY 308  GENETICS              Spring, 2006

INSTRUCTORS:                 Dr. Alix G. Darden 

Dr. Darden’s OFFICE:                       324 Bond  Hall

Dr. Darden’s PHONE:                        953-7873                 E-MAIL: dardena@citadel.edu  (I check regularly)

Dr. Darden’sOFFICE HOURS:         M 2-4,  W 9-11, or by appointment

 

OBJECTIVES: Learn and understand the vocabulary and concepts of molecular and transmission genetics. Be able to apply the vocabulary and concepts of molecular and transmission genetics to problem solving.  Be able to find current, reliable information in the area of genetics.  Be able to perform laboratory exercises, collect and analyze the data. 

 

CLASS POLICY

 

TEXTBOOK: Russell, iGenetics.    My lectures are given with the expectation that you have looked over the material to be covered that day before coming to class. You are responsible for all material in the textbook, even if it has not been gone over in class, unless told otherwise.  This includes knowing the information in the figures and figure legends.  You are also responsible for all lecture material, even if it is not in the textbook.

 

LAB MANUAL:  The lab exercises are on WebCt.  You need to download, print and review the lab exercise before coming to lab.  You need to bring a flowchart of the day’s lab and show this to me to be allowed to START the lab.  The flow chart will also be attached to the lab report.  This will enable you to complete the exercise in the time allowed.  The labs are designed to complement and aid in your understanding of the lecture material.

 

HOMEWORK: HW is assigned during the semester to be handed in and graded.  Each homework is worth 10 points and the eight highest grades are counted.  Homework is designed to keep students focused on critical genetic issues, keeping up with the material and to catch misunderstandings before major tests.  Except for one case, HW is due on Monday’s and will be posted to WebCT by the Friday before, if not sooner.

 

ATTENDANCE:  The attendance policy is that described in the College Handbook.  PLEASE NOTE:  Any student missing more than 20% of the class meetings may be given a grade of F at the instructor’s discretion according to college policy. Absence from more than two (2) laboratory periods will result in a grade of F.   All efforts to attend exams the day they are given should be made.  Students need to speak to me 24 hours before the day the exam is given if a make-up exam is to be considered. A make-up exam contract complete with date and time of make-up will be filled out and signed by student and myself.  Without this make-up contract a student will not be allowed to make-up the exam and will get a grade of “0”.  All make-ups are given before the class next meets.  Make-ups tests are not necessarily the same format as in class exams and may consist of several essay questions

GRADING SYSTEM:          LECTURE – 75%,    LAB – 25% of grade       

Material handed in late losses 10% of the grade per day late, including weekends.  Assignments more than a week late will receive a grade of zero.

Lecture Case Study                                                            40 points

                                Lecture tests (4/5) -                                              400 points (drop lowest test grade)

Comprehensive Final -                                        100 points

Literature searches/society activity (2)            80 points (40 pts each)

Homework (8 highest are counted)                   80 points (10 pts each)

 

Lab -       Lab hand-ins                                                         150 points (6 @ 25 pts)

2 Lab quizzes                                                        150 (75 pts each)

 

Lecture tests will consist of definitions of vocabulary, matching, multiple choice, short answer and problem solving. Vocabulary will be taken from the bolded words in the text and words found in titles.  Late lab reports/literature searches are penalized at 10% grade reduction per day late.  No reports/literature searches are accepted beyond one week after the due date.  Homework will NOT be accepted late.

 

A = 900-1000 points             B = 800-899           C = 700-799           D = 600-699           F = Below 600

 

WebCT: Essential class information can be found on WebCT under Biology 308.  1)All grades will be posted here (“grades”), so check to make sure I don’t make mistakes. 2) All labs are on WebCT and will need to be downloaded before coming to lab found under “Lab Manual”.  3) Data generated in lab will be posted on WebCT under “Data”.  4) HW will be posted on WebCT under “Homework”. 5) The class calendar contains all due dates in an easy to read format under “Calendar”.  6)Past tests can be found as well as study guides for each test under “Past Tests” and “Study Guides”. 


Genetics - Biology 308 Spring 2006 Schedule

 

 

Week

Lecture

Dates

Chap/Topic

Lab exercise

Assignments due

LR=lab report

1

1/11

1-Intro – pp 1-12

None

 

1/3

2-pp.13-38

 

2

1/18

2-

1)Intro to electrophoresis

2) creating lab flowcharts

3) micropipette review

HW#1

1/20

3-pp. 43-62

 

3

1/23

3-

Cancer case study – bibliographic database use – meet in library

HW#2

1/25

4 – pp. 67-73(top) 77-79(top)

 

1/27

Test 1- chap 2,3,4, lab 1

Test 1

4

1/30

5-pp. 87-108, skim 102-106

Cloning 1 - restriction digest, gel electrophoresis

HW#3

2/1

5

Case study write-up

Lab: flow chart

2/3

5

 

5

2/6

6-pp 111-128

Cloning 2 – gel elution and ligation

HW#4

2/8

6

Lab: 1)LR1 – cloning 1 (req)

2) Flow chart

2/10

7/no class

 

6

2/13

Test 2 – chap 5,6

1) Cloning 3 – bacterial transformation

2) Plant phylogeny PCR

Test 2

2/15

7-pp133-166

1)HW#5,

Lab: 1)LR2 – cloning 2 (req)

2) Flowchart

2/17

7

 

7

2/20

7

1) Cloning 4 – plasmid mini-prep

2)Plant phylogeny analysis

HW#6

2/22

8-pp175-204

Lab: 1) LR3-cloning 3 (req)

2)Flow chart

2/24

8

 

8

2/27

8

1)Cloning 5 – final gel analysis – cloning lab review

2)Statistics review

HW#7

3/1

11-pp271-291

Lab: Flow chart

Mid-Term grading

 

3/3

Test 3 – 7,8

Test 3

9

3/6

11

Lab quiz #1 - cloning experiment and electrophoresis

HW#8

3/8

12 –pp. 299-330

Last day to withdraw with “W”

Lab: 1)LR4–cloning final (req)

2) lab quiz

3/10

12

 

10

3/13

12

Human DNA fingerprinting – part 1

HW#9

3/15

13-337-354

Lit search #1

Lab: Flow chart

3/17

13

 

11

3/20

13

Human DNA fingerprinting –part 2

HW#10

3/22

15-pp 412-420, 430-433

Lab: review last weeks flow chart

3/24

Test 4 – chap 11,12,13

Test 4

*********Spring Break************

12

4/3

15

Bioinformatics – meet in computer lab – Bond 251

 

4/5

18-pp 441 – 454

Lab: 1) LR 5 – DNA fingerprinting

2) Flow chart

4/7

18

 

13

4/10

18

Genetically modified food –part 1

HW#11

4/12

19-pp. 515-528

Lab: 1)LR 6 – Bioinformatics

2) Flow chart

4/14

19

 

14

4/17

20-pp. 543-568

GM food part 2

Lab quiz #2

HW#12

4/19

20

Lit search #2 (GM food)

Lab: review last weeks flow chart

4/21

20

 

15

4/24

Test 5 – chap 15,18-20

none

Test 5

LR 7 - GM Food lab report

Sat – 4/29, 8-11    Cumulative Final Exam

 

 

 

Textbook Reading and Suggested Practice Problems

 

 

Reading and Suggested practice problems from the back of each chapter in Russell, iGenetics.  Practice problems will not be collected and corrected.  It is up to you to come to me if you are having difficulties with the problems.  There is often time during lab to go over problems as a group.  Problems similar to the suggested practice problems will appear on exams.

 

Chapter            Pages (# of lectures)                 Suggested practice problems

 

Chap 1 –          1-12(1)                                   

Cha