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2003-2005
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Dr. Alix Darden, Major
Associate Professor
Department of Biology

The Citadel
171 Moultrie Street
Charleston, SC 29409

office: 324 Bond Hall
phone: (843) 953-7873
FAX: (843) 953-7264
email: alix.darden@citadel.edu

Dr. Alix Darden

Courses Taught:

Undergraduate

  • General Biology (BIOL 101)
  • General Biology (BIOL 102)
  • Genetics (BIOL 308) S06
  • Molecular Genetics (BIOL 424)
  • Immunology (BIOL 427)

Graduate

  • Genetics (BIOL 508)
  • Molecular Genetics (BIOL 624)

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Degrees:

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Research Interests:

  • Science Education: I am interested designing learning activities which promote the development of abstract thinking in both traditional and non-traditional students. As a 2000-2001 Carnegie Scholar I learned appropriate techniques to approach this research problem in a scholarly, systematic fashion.
  • Vision research: I am studying the structural and functional properties of salamander and Xenopus opsins and their promoters. This research is done predominantly by undergraduate and graduate biology education students and involves non-radioactive library screening, basic cloning techniques, eukaryotic cell transfection, immunohistochemistry and creating transgenic xenopus.

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Publications:

Chapter in book:

  • Darden, A.G., J.W. Streilein. 1986. Can a mammalian species with monomorphic class I MHC molecules succeed, in G.W. Hoffmann, J.G. Levy, G.T. Nepom (Ed.), Paradoxes in Immunology, (pgs. 20-26). Boca Raton: CRC Press.

Selected Publications (* after number indicates work done at The Citadel):

  • Deysine M, Chua A, Gerboth A: Selective delivery of antibiotics to experimental infection by autologous white blood cells. Surg Forum 30:38-39, 1979. 2.
  • Stein-Streilein J, Lipscomb MF, Hart DA, Darden A: Graft-versus-host reaction in the lungs. Transplantation 32: 38-44, 1981.
  • Darden AG, Streilein JW: Syrian hamsters possess class I-like molecules detectable with xenoantisera but not alloantisera. Transplantation Proc 15: 145-148, 1983.
  • Darden AG, Streilein JW: Lack of Class I polymorphism in Syrian hamsters: Analyses using alloantisera and xenoantisera. Transplantation Proc 15: 1565-1566, 1983.
  • Streilein JW, Darden AG, Phillips JT: Primordial MHC function may be best served by monomorphism. Immunol Today 5: 87-88, 1984.
  • Darden AG, Streilein JW: Syrian hamsters express two class I major histocompatibility complex molecules, neither of which is polymorphic. Immunogenetics 20: 603-622, 1984.
  • Darden AG, Streilein JW: Syrian hamsters possess monomorphic class I molecules. Transplantation Proc 17: 980-981, 1985.
  • Darden AG, Streilein JW: Identification of monomorphic MHC class I molecules in the Syrian hamster. Transplantation Proc 17: 1818-1819, 1985.
  • Darden AG, Streilein JW: Can a mammalian species with monomorphic class I MHC molecules succeed? in Paradoxes in Immunology, eds. G.W. Hoffmann, J.G. Levy, G.T. Nepom, CRC Press, Boca Raton, p. 20-26, 1986.
  • Forbes RDC, Parfrey NA, Gomersall M, Darden AG, Guttmann RD: Dendritic cell-lymphoid cell aggregation and major histocompatibility antigen expression during rat cardiac allograft rejection. J Exp Med 164: 1239-1258, 1986.
  • Darden AG, Forbes RDC, Parfrey NA, Darden PM, Guttmann RD: Genetics of Ia+ interstitial dendritic cells in the heart and pancreas of inbred and congenic rats. Transplantation Proc 14: 208-209, 1986.
  • Darden AG, Forbes RDC, Guttmann RD: Rat cardiac dendritic cells: Frequency in inbred and congenic rats. Transplant Proc 19(1):208, 1987.
  • Forbes RDC, Darden AG, Gomersall M, Parfrey NA, Guttmann RD: Evidence for dendritic cell-lymphocyte clustering in vivo. Transplantation Proc 19:195-197, 1987.
  • Forbes RDC, Darden AG, Gomersall M, Guttmann RD: Profiles of donor MHC antigen expression in rat cardiac allograft rejection. Transplantation Proc 19:3454-3458, 1987.
  • Forbes RDC, Lowry RP, Darden AG, Gomersall M, Marghesco DM: Morphologic studies of acute rat cardiac allograft rejection across an isolated major histocompatibility complex class I (RT1A) disparity. Transplantation 45:943-948, 1988.
  • Forbes RDC, Darden AG, Gomersall M, Guttmann RD: Patterns of donor major histocompatibility complex antigen expression in rat heart grafts and a model of chronic vascular rejection. Transplant Proc 21(1):447-448, 1989.
  • Darden AG, Forbes RDC, Darden PM, Guttmann RD: The effects of genetics and age on expression of MHC class II and CD3 antigens on cardiac interstitial dendritic cells. Cell Immunol 126(2):322-330, 1990.
  • Forbes RD, Gomersall M, Darden AG, Guttmann RD: Multiple patterns of MHC class II antigen expression on cellular constituents of rat heart grafts. Lack of correlation with graft survival, but strong correlation with vasculitis. Transplantation 51(5):942-948, 1991.
  • *Darden AG, Ries WL, Wolf WC, Rodriquiz RM, Key LL: Osteoclastic superoxide production and bone resorption: Stimulation and inhibition by modulators of NADPH oxidase. Journal Bone and Mineral Research 11(5):671-675. 1996.
  • *Darden AG. Assessing Classroom Research. Focus on Microbiology Education 7(1):4-6. 2001
  • *Joel Gramling and Alix Darden, Using Models in the Classroom and Measuring Their Effectiveness. In: The Proceedings of First Annual Charleston Connections: Innovations in Higher Education Conference. June, 2001
  • *Laser, M, Li, Y, Wu, BX, Othersen, KL, Hazard, SE, Darden, AG, Xu, L, Crosson, C, Ma, J. A mammalian homologue of the yeast autophagocytosis protein is induced by ischemic precondition in the retina. J Biol Chem. Submitted.
  • *Darden, AG. Comparison of SOTL to scholarship of genomics. ASM News, 68(3):152-153.
  • Darden, A.G., B.X. Wu, S.L. Znoiko, E.S. Hazard, III, M. Kono, R.K. Crouch, J.X. Ma. 2003. A novel Xenopus SWS2, P434 visual pigment: structure, cellular location, and spectral analysis. Molecular Vision. 9:191-9.

Abstracts and Presentations (* after number indicates work done at The Citadel):

  • Deysine M, Chua A, Gerboth A: Kanamycin bound to a carrier selectively delivered intravenously to a target abscess by autologous white cells. Clin Res 27: A343, 1979.
  • Chua A, Deysine M, Gerboth A: Kanamycin bound to a carrier selectively delivered intravenously to a target abscess by autologous white cells. Crit Care M 7: 189, 1979.
  • Deysine M, Chua A, Gerboth A: Kanamycin bound to a carrier selectively delivered intravenously to a target abscess by autologous white cells. Fed Proc 38: 429, 1979.
  • Darden AG, Streilein JW: Evidence for a non-polymorphic class I major histocompatibility locus in the Syrian hamster. 2nd congress of the International Society of Developmental and Comparative Immunology. Plenum Press, 1981.
  • Gerboth A, Streilein JW: Analysis of Syrian hamster MHC using xenoantisera. Fed Proc 40: 1052, 1981.
  • Darden AG, Streilein JW: Analysis of Syrian hamster MHC using xenoantisera. Amer Zool 21: 9755, 1981.
  • Darden AG, Streilein JW: Syrian hamsters possess class I-like molecules detectable with xenoantisera but not alloantisera. IX. International Congress Transplantation Soc S6.3, 1982.
  • Darden AG, Streilein JW: Lack of class I polymorphism in Syrian hamsters. Analyses using alloantisera and xenoantisera. Rat Histocompatibility Workshop, 1982.
  • Darden AG, Streilein JW: Structural evidence for Syrian hamster class I molecules. Amer Zool 22: 869, 1982.
  • Darden AG, Streilein JW: Syrian hamsters possess monomorphic class I molecules. X. International Congress Transplantation Soc, 1984.
  • Darden AG, Streilein JW: Identification of monomorphic MHC class I molecules in the Syrian hamster. Rat Histocompatibility Workshop, 1984.
  • Darden AG, Forbes RDC, Parfrey NA, Guttmann RD: Genetics of Ia+ interstitial dendritic cells in the heart and pancreas of inbred and congenic rats. 6th International Congress of Immunology, Toronto, 1986.
  • Darden AG, Forbes RDC, Guttmann RD: Rat cardiac dendritic cells: Frequency in inbred and congenic rats. XI International Congress Transplantation Society, 1986.
  • Forbes RDC, Parfrey NA, Gomersall M, Darden AG, Guttmann RD: Modulation of donor MHC antigen expression during rat cardiac allograft rejection. XI International Congress Transplantation Society, 1986.
  • Darden AG, Wolf WC, Ries WL. Key LL: Inhibition of superoxide production in osteoclasts by diphenyl iodonium chloride. American society of Bone and Mineral Research, Tampa Bay, Florida. J Bone Min Res, 8s:s1106. 1993.
  • Darden AG, Oexmann MJ, Hatcher HC, Key LL: Increased survival resulting from nutritional intervention in the microphthalmic model of osteopetrosis. American Society of Bone and Mineral Research, Tampa Bay, Florida. J Bone and Mineral Res, 8s:s697. 1993.
  • Darden AG, Oexmann MJ, Hatcher HC, Key LL: Increased survival resulting from nutritional intervention in the microphthalmic model of osteopetrosis. General Clinical Research Centers Program, Reston, VA, 1993.
  • Darden AG, Oexmann MJ, Key LL: Increased longevity of osteopetrotic microphthalmic mouse by dietary intervention. J Bone and Mineral Res, 9s:s348. 1994.
  • Key LL, Ries WL, Rodriguiz R, Darden AG: Interferon gamma stimulates bone resorption in the microphthalmic mouse via production of superoxide by NADPH oxidase. J Bone and Mineral Res, 9s:s176. 1994.
  • *Darden AG, Oexmann MJ, Wentz B, Key LL: Infertility of the mi/mi Osteopetrotic mouse overcome by dietary intervention. Biology of Reproduction, 54s1:137, 1996.
  • *Darden AG, Garcia SA, Demos C, Spryropoulos DD: Analysis of single and double knockout mutations in the mouse HoxC5 and HoxC6 genes. Presented at Student Research Day, Medical University of South Carolina, 1996.
  • *Darden AG, Garcia SA, Demos C, Spryropoulos DD: Analysis of HoxC5 and HoxC6 single knock-out mutants and HoxC5-homeoboxdefective/HoxC6 knockout double mutants in the mouse. Presented at: The Sixth South Carolina Statewide Research Conference. Isle of Palms, SC, 1997.
  • *Garcia-Gasca A, Darden A, Spyropoulos D, Capecchi M. A classic homeotic transformation in a Hoxc6 knockout mouse is rescued by the addition in cis of a mutation in Hoxc5. Presented at: European Molecular Biology Conference on Mouse Molecular Genetic Meeting. Heidelberg, Germany, 1997.
  • *A.G. Darden and E.Carter. Searching the literature for credit and fun. American Society for Microbiology Annual Meeting. Chicago, IL, 1999.
  • *Alix G. Darden and Elizabeth W. Carter. Using Literature searches and abstract reading to reinforce the concept of the scientific method. Gordon Research Conference, Undergraduate Microbial Education. New London, CT, 1999.
  • *Elizabeth W. Carter and Alix G. Darden. Beyond the Textbook: Library Instruction in the Primary Science Literature. Annual Conference of the American Library Association. New Orleans, LA. 1999.
  • *Wu, BX, ES Li, ES Hazard, A Darden, CE Crosson, JX Ma. PC3-96, A novel mammalian autopphagy gene induced by ischemic preconditioning in the retina. Investigative Ophthalmology and Visual Science, 42(4):S746. 2001.
  • *Mabrouk, S and A Darden. From the classroom and beyond: The scholarship of teaching and learning. 2001 Annual Meeting of Southestern Section of The American Society for Engineering Education. Charleston, SC. 2001.
  • *The Citadel. Many Builders….One CASTLE. The Citadel Academy for the Scholarship of Teaching, Learning and Evaluation. Poster presentation at: AAHE 2001 National Conference on Higher Education Colloquium on Campus Conversations. Washington, DC. 2001.
  • *Darden, AG, D Fallon, S Mabrouk, B Zaremba. A Community of Teaching and Learning Scholars. Workshop presentation at: AAHE 2001 National Conference on Higher Education Colloquium on Campus Conversations. Washington, DC. 2001.
  • *Joel Gramling and Alix Darden, Using Models in the Classroom and Measuring Their Effectiveness. First Annual Charleston Connections: Innovations in Higher Education Conference. Charleston, SC, 2001.
  • *B. Wu, AG Darden and J-X Ma. Cloning and characterization of a novel human autophagy gene that may play a role in tumorigenesis. Hollings Cancer Center Medical University of South Carolina, First Annual Research Retreat. Wild Dunes, SC, 2001.
  • *Darden, AG. Designing a research-based course using Genome Consortium for Active Teaching (GCAT) resources. American Society for Microbiology Annual Meeting. Salt lake City, Utah, 2002.
  • *Darden, AG. Evolution of a Classroom Research Project: It’s all About Student Learning. Second Annual Charleston Connections: Innovations in Higher Education Conference. Charleston, SC, 2002.
  • *Darden, AG. From a student learning difficulty to a teaching technique to a classroom research project: Evolution of a SoTL project. AAHE 2001 National Conference on Higher Education Colloquium on Campus Conversations. Chicago, IL, 2002.
  • *Hill, A, Darden, AG, Ma, JX. Isolating the tiger salamander green rod photoreceptor gene. Summer undergraduate research presentations. Medical University of South Carolina. 2002.
  • *Shillinglaw, S, Darden, AG, Ma, JX. Isolation of the salamander red rod rhodopsin gene from salamander genomic library. Summer undergraduate research presentations. Medical University of South Carolina. 2002.

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